Cellular Imaging in Four Dimensions 

• 20 hrs lecture
• 60 hrs individual study period

Students gain knowledge into the fundamentals and applications of advanced cellular imaging techniques in biomedical research. The lectures will provide the students with practical examples of ongoing research in the Molecular Life Sciences.

'Cellular Imaging in Four Dimensions; principles and applications' (Coordinator P. Willems, , 3614589)

I. Principles of fluorescence and electron microscopy 

1. Introduction to microscopy (Jack Fransen) 
2. Advanced microscopical techniques (Jack Fransen/Peter Friedl)
3. Proteinacious reporter molecules (Werner Koopman) 
4. Chemical reporter molecules (Peter Willems) 

II. Image processing and quantification in microscopy 

5. Image processing and quantification (Werner Koopman)

6. Analysis of molecular complexes in cellular signal transduction (Roland Brock) 

III. State-of-the-art applications in biology and medicine 

7. Imaging of intracellular protein routing in health and disease (Jack Fransen) 
8. 'Dyeing' mitochondrial shape and function in metabolic disease (Peter Willems/Werner Koopman)

9. Dynamic imaging of cancer (Peter Friedl)

10. Analysis of cellular import, residence time and breakdown of potential therapeutic molecules (Roland Brock)

Examination (Tentaminering)

The final written exam includes 10 assay questions, one question per subject.

Extra information (Bijzonderheden)

contact: mrs L. Brocatus, 024-3614259,

The final written exam includes both parts of the course.

contact: mrs. J. Rullmann, 3652701,